This course, to study biological samples from nanometric to cell and organoids imaging, is intended for students who will be doing cell biology in the laboratory.
The latest technologies (optogenetics, AFM, membrane biology, FRET/FRAP, etc.) will be presented theoretically and practically.
Fluorescent Probes (Sylvain Féliciangeli, IPMC)
Photomanipulation FRAP, FRET, FLIM and Applications (Caroline Medioni, IBV)
Lipids and membrane dynamics in imaging (Bruno Mesmin, IPMC)
"Atomic force microscopy: the blind microscope with a tactile vision for probing the nanoscale world" (Sabrina Pisano, IRCAN)
Biological models : Drosophila, Mice, C. elegans, cells,...
AFM Core-facility-Sabrina Pisano: Membrane forces in physiology and pathology
IBV - imaging-facility -Sameh Ben Aicha: 3D Imaging of Neural Organoids Using Light Sheet Microscopy: From Sample Preparation to Image Analysis.
IBV - Guillaume Sandoz's lab: Reversible optical control of pain in freely moving animals. Photopharmacology is an emerging technique that uses light-responsive molecules to control the activity of receptors and ion channels with high spatiotemporal precision given by the beam of light.
IPMC - imaging-facility and optogenetic: Sylvain Feliciangeli: photoactivation and imaging
IPMC - cytometry-facility- Julie Cazareth/Sophie Abelanet: cytometry and immunophenotyping
C3M- imaging-facility- Jérôme Gilleron : Functional exploration of endocytic trafficking (Rab protein).
Scientific articles taken from recent literature will be studied.
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