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UE Life imaging

Code de l'ECUE : SMUSVPK

Ce cours donne droit à 3.0 ECTS.
EUR LIFE
Neurosciences , Biologie cellulaire , Physiologie
Campus Valrose
Master 1 , Master 2
Semestre pair
Anglais

PRESENTATION

This course, to study biological samples from nanometric to cell and organoids imaging, is intended for students who will be doing cell biology in the laboratory.

The latest technologies (optogenetics, AFM, membrane biology, FRET/FRAP, etc.) will be presented theoretically and practically.

 

Responsable(s) du cours

, Caroline Medioni

Présentiel

PREREQUIS

Avant le début du cours, je dois ...
  • Enjoy cell biology
  • Have a bachelor's degree in life sciences

OBJECTIFS

A la fin de ce cours, je devrais être capable de...
  • Know the main uses and characteristics of the devices used
  • Choosing an imaging technique adapted to the biological question to be resolved
  • Read a scientific article in English, present it orally and write a summary
  • Know the basics of the main methods of observation, analysis, and exploration in cell biology
  • To emphasize the interest of the choice of technique according to the biological question.

CONTENU

  • Lipids and membrane dynamics in imaging (Bruno Mesmin, IPMC)

    Techniques de Photomanipulation FRAP, FRET, FLIM et Applications (Caroline Medioni, IBV)

    Study of biological samples for nanometric imaging, and/or for the study of their mechanical properties (Sabrina Pisano, IRCAN)

    Fluorescent tools (Alain Burger, ICN)

    Biological models : Drosophila, Mice,...

  • AFM Core-facility

    IBV - imaging-facility and optogenetic

    IPMC - cytometry-facility

    C3M- imaging-facility

     

  • Scientific articles taken from recent literature will be studied.

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Important
Ce syllabus n’a aucune valeur contractuelle. Son contenu est susceptible d’évoluer en cours d’année : soyez attentifs aux dernières modifications.